Fall 2024

     During my time in the Jankowski Lab, I was able to refine my techniques in bench lab, while understanding more about the project I am completing. I am exploring pain management and focusing on ischemic myalgia, a form of muscle pain due to the onset of a reperfusion injury. Our project focuses on the correlation between the presence of CCL27A, a chemokine ligand, and the T-cell count within the I/R injured muscle cells. 

     My main purpose shifted from learning and practicing lab techniques to implementing said techniques for our project. I was able to perform my first procedures on the lab mice, such as administering a reperfusion injury and harvesting the dorsal-root ganglion from the spine. I used RNAscope technology to analyze and quantify RNA in muscle cell and tissue. I performed the ELISA technique and qPCR to measure DNA and enzyme activity. I was able to piece together how each portion of the experiment helped to develop specific results we could analyze.

     We were able to conclude majority of the experiments for this project, and are shifting gears towards writing the paper for publication. I cannot wait to see how this project flourishes!